| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 10B Chupacabras |
0443-01SLB3U-0006 | Aaron Alva |
| A2 | Tennessee Soccer Club TSC 2010 Boys Nike |
0522-011B3U-0110 | Lee Hudson |
| A3 | Stones River Futbol Club - 01 Stones River FC 2010 Red |
0521-012B3U-2083 | Ahmed Meho |
| A4 | Chattanooga FC Academy Chattanooga FC - 2010B Navy |
0316-022B3U-7880 | Richard Dixon |
| A5 | Clarksville Soccer Club Clarksville SC - B2010 Maroon |
0422-013B3U-0008 | Alejandro Herrera |
| A6 | Armada Futbol Club Armada FC 10B Red |
0436-012B3U-0046 | Mohamed Adan |
| A7 | Tri-Cities United Soccer Club Tri-Cities United 2010 Boys Premier |
0106-011B3U-2243 | O Chirica |
| A8 | Lobos Rush (Collierville Soccer Association) 2010 Lobos Rush Elite |
0603-012B3U-0035 | Katie Smith |