| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Wilson United Soccer Club WUSC - 2009 Boys Black |
0406-013B4U-0005 | Christian Trujillo |
| A2 | Johnson City Futbol Club - 01 JCFC 2009B |
0216-012B4U-0104 | Dan Trull |
| A3 | Lobos Rush (Collierville Soccer Association) 2009 Lobos Rush Elite |
0603-012B4U-0036 | Christopher Wright |
| A4 | All-In FC TN All-In FC TN 09B Chippewas |
0443-01SLB4U-0007 | Davis Draper |
| A5 | Emerald Force Soccer Club - 01 Emerald Force 09 Manchester City |
0214-012B4U-1246 | Robert Gibson |
| A6 | Stones River Futbol Club - 01 Stones River FC 2009 Red |
0521-012B4U-2084 | Fulgence Letema |
| A7 | Tennessee Soccer Club TSC Williamson 2009 Boys Showcase |
0522-012B4U-0191 | Ruben Arcega |
| A8 | Nashville United Soccer Academy - 01 NUSA B09 Navy |
0534-012B4U-0020 | Thomas Campbell |